Executive Summary The aim of the report is to eval Original Essay Help

Executive Summary The aim of the report is to eval Original Essay Help

DESSERTATION PROJECT PROTOCOL INTRODUCTION A1. Hydrogen peroxide which is used as food additives (ROS) and toxicological consideration of H2O2. 7-8 pages good reviews. How much hydrogen peroxide reaches to colon? A2. How hydrogen peroxide absorb in the body and dose? A3. What concentration has been used in the food? A4. Does hydrogen peroxide found in food cytotoxic? A5. About Caco2 cells A6 Hypothesis B.METHOD B1 Tissue culture C.AIM D.RESULT It should be calculate dose response in result. E.DISCOSSION Should be 3000 word F.CONCLUSION G.ABSTRACT NOTES I did approximately 3678 word but I need 6500 word more. H2O2 treated with Caco2 cells Commercial hydrogen peroxide was used as a 9.79 Moles and intermediate dilation was carried out during the preparing concentration of hydrogen peroxide. Hydrogen peroxide concentrations 0.5 ml H2O2 + 4.5 ml PBS has been arrange for 1 Moles (hydrogen peroxide concentration). 3.3 ml has been taken from previous concentration + 1.7 ml PBS for 0.66 Moles (hydrogen peroxide concentration). 3.33 ml previous concentration +1.67 ml PBS for 0.44 Moles (hydrogen peroxide concentration) 3.29 ml previous +1.71ml PBS for 0.29 Moles (hydrogen peroxide concentration) 3.44 ml previous + 1.55 ml PBS 0.20 Moles (hydrogen peroxide concentration) 3.25 ml previous + 1.75 ml PBS 0.13 Moles (hydrogen peroxide concentration) 3.07 ml previous + 1.93 PBS 0.08 Moles (hydrogen peroxide concentration) This MTT assay repeated 3 times. Because of there are 3 results. Control Just cell 0.08 moles 013 moles 0.20 moles 0.29 moles 0.44 moles 0.66 moles 1 moles Blank (no cell) 0.913 0.253 0.228 0.197 0.221 0.188 0.193 0.114 0.151 0.957 0.351 0.232 0.227 0.201 0.238 0.223 0.189 0.164 1.099 0.227 0.214 0.201 0.176 0.142 0.151 0.108 0.168 1.193 0.214 0.35 0.252 0.243 0.212 0.216 0.198 0.173 0.915 0.236 0.216 0.239 0.229 0.202 0.22 0.199 0.173 0.96 0.203 0.221 0.214 0.219 0.203 0.213 0.198 0.197 0.984 0.225 0.212 0.207 0.224 0.202 0.207 0.199 0.171 1.11 0.238 0.217 0.228 0.234 0.208 0.21 0.25 0.172 Table 1: Plate 1. MTT Absorbance Caco2 cells treated with hydrogen peroxide at 540 nm Concentration (Moles) Average Standard Deviation CV(COEFFIENT OF VARI Control (Just cell) 1.016375 0.130857 0.102184 0.08 Moles 0.243375 0.046071 0.189301 0.13 Moles 0.23625 0.046478 0.196733 0.20 Moles 0.2200625 0.019235 0.087184 0.29 Moles 0.218375 0.021044 0.096365 0.44 Moles 0.199375 0.027187 0.13636 0.66 Moles 0.204125 0.023351 0.114395 1 Moles 0.181875 0.0447604 0.261739 Blank (No cell) 0.171125 0.012778 0.074668 Table 2: Average, SD, and coefficient of variation of plate 1 Control Just cell 0.08 moles 0.13 moles 0.20 moles 0.29 moles 0.44 moles 0.66 moles 1 moles Blank (no cell) 0.405 0.225 0.214 0.217 0.22 0.198 0.203 0.184 0.225 0.735 0.243 0.219 0.26 0.31 0.219 0.194 0.192 0.225 0.801 0.204 0.214 0.225 0.195 0.197 0.209 0.193 0.229 0.864 0.231 0.252 0.237 0.184 0.195 0.186 0.199 0.261 0.91 0.215 0.245 0.228 0.235 0.222 0.225 0.203 0.235 0.72 0.231 0.241 0.265 0.223 0.232 0.242 0.206 0.227 0.769 0.218 0.236 0.22 0.224 0.195 0.2 0.205 0.217 0.68 0.198 0.228 0.213 0.203 0.206 0.18 0.185 0.232 Table 3: plate 2 MTT absorbance Caco2 cells treated with hydrogen peroxide at 540 nm Concentration (moles) Average Standard deviation Coefficient of variation (CV) Control (just cell) 0.7355 0.153495 0.208695 0.08 moles 0.220625 0.014937 0.067705 0.13 moles 0.231125 0.014604 0.063185 0.20 moles 0.233125 0.019577 0.083977 0.29 moles 0.22425 0.038574 0.172012 0.44 moles 0.208 0.014422 0.069338 0.66 moles 0.204875 0.020427 0.099705 1 moles 0.195875 0.008692 0.044376 Blank 0.231375 0.013114 0.056679 Table 4: Averages, and coefficient of variation of plate2 Control just cell 0.08 moles 0.13 moles 0.20 moles 0.29 moles 0.44 moles 0.66 moles 1 moles Blank 0.757 0.215 0.208 0.2 0.203 0.196 0.2 0.194 0.198 0.92 0.229 0.193 0.214 0.204 0.181 0.179 0.174 0.196 0.861 0.196 0.2 0.205 0.185 0.186 0.196 0.173 0.205 0.868 0.2 0.211 0.209 0.2 0.195 0.189 0.181 0.2 0.938 0.201 0.212 0.228 0.228 0.19 0.169 0.192 0.204 0.86 0.194 0.208 0.234 0.219 0.189 0.192 0.179 0.217 0.786 0.175 0.214 0.192 0.192 0.171 0.153 0.165 0.19 0.787 0.231 0.234 0.209 0.221 0.19 0.179 0.193 0.205 Table 5:Plate3 MTT absorbance Caco2 cells treated with hydrogen peroxide at 540 nm Concentration Averages Standard Deviation Coefficient of variation Control 0.847125 0.065287 0.077069 0.08 moles 0.205125 0.018879 0.092036 0.13 moles 0.21 0.011916 0.056745 0.20 moles 0.211375 0.013897 0.065745 0.29 moles 0.2065 0.014938 0.079776 0.44 moles 0.18725 0.008102 0.043269 0.66 moles 0.182125 0.01557 0.085488 1 moles 0.181375 0.01073 0.059157 Blank 0.201875 0.00799 0.039579 Table 6: Averages, and coefficient of variation of plate3


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